What happened? Alcohol, memory blackouts, and the brain.

Alcohol primarily interferes with the ability to form new long-term memories, leaving intact previously established long-term memories and the ability to keep new information active in memory for brief periods. As the amount of alcohol consumed increases, so does the magnitude of the memory impairments. Large amounts of alcohol, particularly if consumed rapidly, can produce partial (i.e., fragmentary) or complete (i.e., en bloc) blackouts, which are periods of memory loss for events that transpired while a person was drinking. Blackouts are much more common among social drinkers--including college drinkers--than was previously assumed, and have been found to encompass events ranging from conversations to intercourse. Mechanisms underlying alcohol-induced memory impairments include disruption of activity in the hippocampus, a brain region that plays a central role in the formation of new autobiographical memories.

I f recreational drugs were tools, alcohol memories for events that transpire while ing from sensory memory (which lasts up would be a sledgehammer. Few a person is intoxicated, a type of impairment to a few seconds) to short-term memory cognitive functions or behaviors known as a blackout. This article reviews (which lasts from seconds to minutes escape the impact of alcohol, a fact that what is currently known regarding the depending upon whether the information has long been recognized in the literature. specific features of acute alcohol-induced is rehearsed) to long-term storage. This As Fleming stated nearly 70 years ago, memory dysfunction, particularly alcohol-model often is referred to as the modal "the striking and inescapable impression induced blackouts, and the pharmaco-model of memory, as it captures key elements one gets from a review of acute alcoholic logical mechanisms underlying them.
of several other major models. Indeed, intoxication is of the almost infinite elements of this model still can be seen in diversity of symptoms that may ensue virtually all models of memory formation.
from the action of this single toxic agent" Effects of Alcohol on (1935) (pp. 94-95). In addition to Memory AARON M. WHITE, PH.D., is an assistant impairing balance, motor coordination, research professor in the Department of decisionmaking, and a litany of other To evaluate the effects of alcohol, or Psychiatry, Duke University Medical functions, alcohol produces detectable any other drug, on memory, one must Center, Durham, North Carolina. memory impairments beginning after first identify a model of memory formajust one or two drinks. As the dose tion and storage to use as a reference. This work was supported by National increases, so does the magnitude of the One classic, often-cited model, initially Institute on Alcohol Abuse and Alcoholism memory impairments. Under certain proposed by Atkinson and Shiffrin (1968), grant AA-12478 and the Institute for circumstances, alcohol can disrupt or posits that memory formation and stor-Medical Research at the VA Medical completely block the ability to form age take place in several stages, proceed-Center in Durham, North Carolina.
In the modal model of memory, when one attends to sensory informa tion, it is transferred from a sensory memory store to short-term memory. The likelihood that information will be transferred from short-term to longterm storage, or be encoded into longterm memory, was once thought to depend primarily on how long the person keeps the information active in shortterm memory via rehearsal. Although rehearsal clearly influences the transfer of information into long-term storage, it is important to note that other factors, such as the depth of processing (i.e., the level of true understanding and manipula tion of the information), attention, moti vation, and arousal also play important roles (Craik and Lockhart 1972;Otten et al. 2001;Eichenbaum 2002). 1 Variability in the use of terms, par ticularly in operational definitions of short-term memory, makes it difficult to formulate a simple synopsis of the literature on alcohol-induced memory impairments. As Mello (1973) stated three decades ago with regard to the memory literature in general, "The inconsistent use of descriptive terms has been a recurrent source of confusion in the 'short-term' memory literature and 'short-term' memory has been variously defined as 5 seconds, 5 minutes, and 30 minutes" (p. 333). In spite of this inconsistency, several conclusions can be drawn from research on alcohol-induced memory impairments. One conclusion is that the impact of alcohol on the for mation of new long-term "explicit" memories-that is, memories of facts (e.g., names and phone numbers) and events-is far greater than the drug's impact on the ability to recall previously established memories or to hold new information in short-term memory (Lister et al. 1991). (See figure 1 for a diagram depicting the stages of mem ory and where alcohol interferes with memory.) Intoxicated subjects are typi cally able to repeat new information immediately after its presentation and often can keep it active in short-term storage for up to a few minutes if they are not distracted (for an early review, Alcohol and Memory see Ryback 1971), though this is not always the case (Nordby et al. 1999). Similarly, subjects normally are capable of retrieving information placed in longterm storage prior to acute intoxication. In contrast, alcohol impairs the ability to store information across delays longer than a few seconds if subjects are dis tracted between the time they are given the new information and the time they are tested. In a classic study, Parker and colleagues (1976) reported that when intoxicated subjects were presented with "paired associates"-for example, the letter "B" paired with the month "January"-they were impaired when asked to recall the items after delays of a minute or more. However, subjects could recall paired associates that they had learned before becoming intoxicated.
1 It is well beyond the scope of this review to assess the impact of alcohol on memory utilizing multiple perspectives on information processing and storage. For simplicity, this review will characterize the effects of alcohol on memory using a three-stage process of memory formation akin to the modal model. The interpretation of the effects of alcohol on memory likely would vary somewhat depending on the memory model one uses.

Figure 1
A general model of memory formation, storage, and retrieval based on the modal model of memory originally proposed by Atkinson and Shiffrin (1968). Alcohol seems to influence most stages of the process to some degree, but its primary effect appears to be on the transfer of information from short-term to long-term storage. Intoxicated subjects are typically able to recall information immediately after it is presented and even keep it active in short-term memory for 1 minute or more if they are not distracted. Subjects also are normally able to recall long-term memories formed before they became intoxicated; however, beginning with just one or two drinks, subjects begin to show impairments in the ability to transfer information into long-term storage. Under some circumstances, alcohol can impact this process so severely that, once sober again, subjects are unable to recall critical elements of events, or even entire events, that occurred while they were intoxicated. These impairments are known as blackouts.
More recently, Acheson and colleagues (1998) observed that intoxicated sub jects could recall items on word lists immediately after the lists were pre sented but were impaired when asked to recall the items 20 minutes later. Ryback (1971) characterized the impact of alcohol on memory forma tion as a dose-related continuum, with minor impairments at one end and large impairments at the other, all impairments representing the same fundamental deficit in the ability to transfer new information from shortterm to long-term storage. When doses of alcohol are small to moderate (pro ducing blood alcohol concentrations [BACs] below 0.15 percent), memory impairments tend to be small to mod erate as well. At these levels, alcohol produces what Ryback (1971) referred to as cocktail party memory deficits, lapses in memory that people might experience after having a few drinks at a cocktail party, often manifested as problems remembering what another person said or where they were in con versation. Several studies have revealed that alcohol at such levels causes diffi culty forming memories for items on word lists or learning to recognize new faces (Westrick et al. 1988;Mintzer and Griffiths 2002). As the dose increases, the resulting memory impairments can become much more profound, some times culminating in blackouts-periods for which a person is unable to remem ber critical elements of events, or even entire events, that occurred while he or she was intoxicated.

Alcohol-Induced Blackouts
Blackouts represent episodes of amnesia, during which subjects are capable of participating even in salient, emotionally charged events-as well as more mun dane events-that they later cannot remember (Goodwin 1995). Like milder alcohol-induced memory impairments, these periods of amnesia are primarily "anterograde," meaning that alcohol impairs the ability to form new memo ries while the person is intoxicated, but does not typically erase memories formed before intoxication. Formal research into the nature of alcohol-induced blackouts began in the 1940s with the work of E.M. Jellinek (1946).
Jellinek's initial characterization of blackouts was based on data collected from a survey of Alcoholics Anonymous members. Noting that recovering alco holics frequently reported having expe rienced alcohol-induced amnesia while they were drinking, Jellinek concluded that the occurrence of blackouts is a powerful indicator of alcoholism.
In 1969, Goodwin and colleagues published two of the most influential studies in the literature on blackouts (Goodwin et al. 1969a,b). Based on interviews with 100 hospitalized alco holics, 64 of whom had a history of blackouts, the authors posited the exis tence of two qualitatively different types of blackouts: en bloc and fragmentary blackouts. People experiencing en bloc blackouts are unable to recall any details whatsoever from events that occurred while they were intoxicated, despite all efforts by the drinkers or others to cue recall. Referring back to our general model of memory formation, it is as if the process of transferring information from short-term to long-term storage has been completely blocked. En bloc memory impairments tend to have a dis tinct onset. It is usually less clear when these blackouts end because people typi cally fall asleep before they are over. Interestingly, people appear able to keep information active in short-term memory for at least a few seconds. As a result, they can often carry on conversations, drive automobiles, and engage in other complicated behaviors. Information per taining to these events is simply not transferred into long-term storage. Ryback (1970) wrote that intoxicated subjects in one of his studies "could carry on conversations during the amnesic state, but could not remember what they said or did 5 minutes earlier. Their immediate and remote memory were intact" (p. 1003). Similarly, in their study of memory impairments in intoxicated alcoholics, Goodwin and colleagues (1970) reported that subjects who experienced blackouts for testing sessions showed intact memory for up to 2 minutes while the sessions were taking place.
Unlike en bloc blackouts, fragmen tary blackouts involve partial blocking of memory formation for events that occurred while the person was intoxi cated. Goodwin and colleagues (1969a) reported that subjects experiencing frag mentary blackouts often become aware that they are missing pieces of events only after being reminded that the events occurred. Interestingly, these reminders trigger at least some recall of the initially missing information. Research suggests that fragmentary blackouts are far more common than those of the en bloc variety (White et al. 2004;Hartzler and Fromme 2003b;Goodwin et al. 1969b).

Blackouts: State-Dependent Memory Formation?
Early anecdotal evidence suggested that blackouts might actually reflect statedependent information storage-that is, people might be able to remember events that occurred while they were intoxicated if they returned to that state (e.g., Goodwin et al. 1969a). Statedependent memory can be viewed as a special case of a broader category known as context-dependent memory (e.g., White et al. 2002a), in which cues that are associated with an event when a memory is formed tend to help trig ger recall for that event at a later time. For instance, in a classic study by Godden and Baddeley (1975) divers who learned word lists either on land or under water remembered more words when tested in the same context in which learning took place (i.e., land-land or water-water). Likewise, returning to the same emo tional or physiological state that was present when a memory was formed often can facilitate recall of that mem ory. It is not uncommon to hear stories of drinkers who stash alcohol or money while intoxicated and can locate the hiding places only after becoming intoxicated again (Goodwin 1995). Regardless of how compelling such stories can be, clear evidence of statedependent learning under the influence of alcohol is lacking. In one recent study, Weissenborn and Duka (2000) examined whether subjects who learned word lists while intoxicated could recall more items if they were intoxicated again during the testing session. No such state-dependency was observed. Similarly, Lisman (1974) tried unsuccessfully to help subjects resurrect lost information for events occurring during periods of intoxication by getting them intoxi cated once again.

Blood Alcohol Concentrations and Blackouts
Drinking large quantities of alcohol often precedes blackouts, but several other factors also appear to play impor tant roles in causing such episodes of memory loss. As Goodwin and colleagues (1969a) stated with regard to subjects in one of their studies, "Although blackouts almost always were associated with heavy drinking, this alone seemed insufficient to produce one. On many other occasions, subjects said they had drunk as much or more without memory loss" (p. 195). Among the factors that preceded blackouts were gulping drinks and drinking on an empty stomach, each of which leads to a rapid rise in BAC.
Subsequent research provided addi tional evidence suggesting a link between blackouts and rapidly rising BACs. Goodwin and colleagues (1970) examined the impact of acute alcohol exposure on memory formation in a laboratory setting. The author recruited 10 male subjects for the project, all but one through the unemployment office in St. Louis, Missouri. Most subjects met diagnostic criteria for alcoholism and half had a history of frequent blackouts. The men were asked to consume roughly 16 to 18 ounces of 86-proof bourbon in approximately 4 hours. Beginning 1 hour after subjects began drinking, memory was tested by presenting sub jects with several different stimuli, including a series of children's toys and scenes from erotic films. Subjects were asked to recall details regarding these stimuli 2 minutes, 30 minutes, and 24 hours after the stimuli were shown. Half of the subjects reported no recall for the stimuli or their presentation 30 minutes and 24 hours after the events, though most seemed to recall the stim uli 2 minutes after presentation. Lack of recall for the events 24 hours later, while sober, represents clear experimen tal evidence for the occurrence of blackouts. The fact that subjects could remember aspects of the events 2 min utes after they occurred but not 30 minutes or 24 hours afterward provides compelling evidence that the blackouts stemmed from an inability to transfer information from short-term to longterm storage. For all but one subject in the blackout group, memory impair ments began during the first few hours of drinking, when BAC levels were still rising. The average peak BAC in this group, which was roughly 0.28 percent, occurred approximately 2.5 hours after the onset of drinking.
In a similar study, Ryback (1970) examined the impact of alcohol on memory in seven hospitalized alcoholics given access to alcohol over the course of several days. All subjects were White males between the ages of 31 and 44. Blackouts occurred in five of the seven subjects, as evidenced by an inability to recall salient events that occurred while drinking the day before (e.g., one sub ject could not recall preparing to hit another over the head with a chair). Estimates of BAC levels during blackout periods suggested that they often began at levels around 0.20 percent and as low as 0.14 percent. The duration of black outs ranged from 9 hours to 3 days. Based on his observations, Ryback con cluded that a key predictor of blackouts was the rate at which subjects consumed their drinks. He stated, "It is important to note that all the blackout periods occurred after a rapid rise in blood alcohol level" (p. 622). The two subjects who did not black out, despite becom ing extremely intoxicated, experienced slow increases in blood alcohol levels.

Blackouts Among Social Drinkers
Most of the research conducted on blackouts during the past 50 years has involved surveys, interviews, and direct observation of middle-aged, primarily male alcoholics, many of whom were hospitalized. Researchers have largely ignored the occurrence of blackouts among young social drinkers, so the idea that blackouts are an unlikely con sequence of heavy drinking in nonalco-

Alcohol and Memory
holics has remained deeply entrenched in both the scientific and popular cul tures. Yet there is clear evidence that blackouts do occur among social drinkers. Knight and colleagues (1999) observed that 35 percent of trainees in a large pediatric residency program had experi enced at least one blackout. Similarly, Goodwin (1995) reported that 33 per cent of the first-year medical students he interviewed acknowledged having had at least one blackout. "They were inexperienced," he wrote. "They drank too much too quickly, their blood levels rose extremely quickly, and they experi enced amnesia" (p. 315). In a study of 2,076 Finnish males, Poikolainen (1982) found that 35 percent of all males sur veyed had had at least one blackout in the year before the survey.
As might be expected given the excessive drinking habits of many col lege students (Wechsler et al. 2002), this population commonly experiences blackouts. White and colleagues (2002c) recently surveyed 772 undergraduates regarding their experiences with black outs. Respondents who answered yes to the question "Have you ever awoken after a night of drinking not able to remember things that you did or places that you went?" were considered to have experienced blackouts. Fifty-one percent of the students who had ever consumed alcohol reported blacking out at some point in their lives, and 40 percent reported experiencing a black out in the year before the survey. Of those who had consumed alcohol dur ing the 2 weeks before the survey, 9.4 percent reported blacking out during this period. Students in the study reported that they later learned that they had participated in a wide range of events they did not remember, including such significant activities as vandalism, unprotected intercourse, driving an automobile, and spending money.
During the 2 weeks preceding the survey, an equal percentage of males and females experienced blackouts, despite the fact that males drank signif icantly more often and more heavily than females. This outcome suggests that at any given level of alcohol consumption, females-a group infrequently studied in the literature on blackouts-are at greater risk than males for experiencing blackouts. The greater tendency of females to black out likely arises, in part, from well-known gender differences in physiological factors that affect alco hol distribution and metabolism, such as body weight, proportion of body fat, and levels of key enzymes. There also is some evidence that females are more susceptible than males to milder forms of alcohol-induced memory impairments, even when given comparable doses of alcohol (Mumenthaler et al. 1999).
In a subsequent study, White and colleagues (2004) interviewed 50 undergraduate students, all of whom had experienced at least one blackout, to gather more information about the factors related to blackouts. As in the previous study, students reported engag ing in a range of risky behaviors during blackouts, including sexual activity with both acquaintances and strangers, vandalism, getting into arguments and fights, and others. During the night of their most recent blackout, most students drank either liquor alone or in combi nation with beer. Only 1 student out of 50 reported that the most recent blackout occurred after drinking beer alone. On average, students estimated that they consumed roughly 11.5 drinks before the onset of the blackout. Males reported drinking significantly more than females, but they did so over a significantly longer period of time. As a result, estimated peak BACs during the night of the last blackout were simi lar for males (0.30 percent) and females (0.35 percent). As Goodwin observed in his work with alcoholics (1969b), fragmentary blackouts occurred far more often than en bloc blackouts, with four out of five students indicating that they eventually recalled bits and pieces of the events. Roughly half of all students (52 percent) indicated that their first full memory after the onset of the blackout was of waking up in the morning, often in an unfamiliar location. Many students, more females (59 percent) than males (25 percent), were frightened by their last blackout and changed their drinking habits as a result.

Use of Other Drugs During Blackouts
Alcohol interacts with several other drugs, many of which are capable of produc ing amnesia on their own. For instance, diazepam (Valium ® ) and flunitrazepam (Rohypnol) are benzodiazepine seda tives that can produce severe memory impairments at high doses (White et al. 1997;Saum and Inciardia 1997). Alcohol enhances the effects of benzo diazepines (for a review, see Silvers et al. 2003). Thus, combining these com pounds with alcohol could dramatically increase the likelihood of experiencing memory impairments. Similarly, the combination of alcohol and THC, the primary psychoactive compound in marijuana, produces greater memory impairments than when either drug is given alone (Ciccocioppo et al. 2002). Given that many college students use other drugs in combination with alco hol (O'Malley and Johnston 2002), some of the blackouts reported by stu dents may arise from polysubstance use rather than from alcohol alone. Indeed, based on interviews with 136 heavydrinking young adults (mean age 22), Hartzler and Fromme (2003b) concluded that en bloc blackouts often arise from the combined use of alcohol and other drugs. White and colleagues (2004) observed that, among 50 undergraduate students with a history of blackouts, only 3 students reported using other drugs during the night of their most recent blackout, and marijuana was the drug in each case.

Are Some People More Likely Than Others to Experience Blackouts?
In classic studies of hospitalized alcoholics by Goodwin and colleagues (1969a,b), 36 out of the 100 patients interviewed indicated that they had never experi enced a blackout. In some ways, the patients who did not experience black outs are as interesting as the patients who did. What was it about these 36 patients that kept them from blacking out, despite the fact that their alcoholism was so severe that it required hospital ization? Although they may actually have experienced blackouts but simply were unaware of them, there may have been something fundamentally different about these patients that diminished their likelihood of experiencing mem ory impairments while drinking.
In support of this possibility, a recent study by Hartzler and Fromme (2003a) suggests that people with a history of blackouts are more vulnerable to the effects of alcohol on memory than those without a history of blackouts. These authors recruited 108 college students, half of whom had experienced at least one fragmentary blackout in the previous year. While sober, members of the two groups performed comparably in mem ory tasks. However, when they were mildly intoxicated (0.08 percent BAC) those with a history of fragmentary blackouts performed worse than those without such a history. There are two possible interpretations for these data, both of which support the hypothesis that some people are more susceptible to blackouts than others. One plausible interpretation is that subjects in the fragmentary blackout group always have been more vulnerable to alcoholinduced memory impairments, which is why they performed poorly during testing under alcohol, and why they are members of the blackout group in the first place. A second interpretation is that subjects in the blackout group performed poorly during testing as a result of drinking enough in the past to experience alcohol-induced memory impairments. In other words, perhaps their prior exposure to alcohol damaged the brain in a way that predisposed them to experiencing future memory impairments. This latter possibility is made more likely by recent evidence that students who engage in repeated episodes of heavy, or binge, drinking are more likely than other students to exhibit memory impairments when they are intoxicated (Weissenborn and Duka 2000). Similar results have been observed in animal studies (White et al. 2000a).
The argument for an inherent vul nerability to alcohol-induced memory impairments, including blackouts, is strengthened by two recent studies. In an impressive longitudinal study, Baer and colleagues (2003) examined the drinking habits of pregnant women in 1974 and 1975, and then studied alco hol use and related problems in their offspring at seven different time points during the following 21 years. These authors observed that prenatal alcohol exposure was associated with increased rates of experiencing alcohol-related consequences, including blackouts, even after controlling for the offsprings' general drinking habits. In addition, a recent report by Nelson and colleagues (2004) suggests that there might actu ally be a genetic contribution to the susceptibility to blackouts, indicating that some people simply are built in a way that makes them more vulnerable to alcohol-induced amnesia.
As discussed in the section below on the potential brain mechanisms under lying alcohol-induced amnesia, it is easy to imagine that the impact of alcohol on brain circuitry could vary from per son to person, rendering some people more sensitive than others to the memory-impairing effects of the drug.

How Does Alcohol Impair Memory?
During the first half of the 20th century, two theoretical hurdles hampered progress toward an understanding of the mecha nisms underlying the effects of alcohol on memory. More recent research has cleared away these hurdles, allowing for tremendous gains in the area during the past 50 years.
The first hurdle concerned scientists' understanding of the functional neuro anatomy of memory. In the 1950s, following observations of an amnesic patient known as H.M., it became clear that different brain regions are involved in the formation, storage, and retrieval of different types of memory. In 1953, large portions of H.M.'s medial temporal lobes, including most of his hippocam pus, were removed in an effort to con trol intractable seizures (Scoville and Milner 1957). Although the frequency and severity of H.M.'s seizures were sig nificantly reduced by the surgery, it soon became clear that H.M. suffered from a dramatic syndrome of memory impairments. He still was able to learn basic motor skills, keep information active in short-term memory for a few seconds or more if left undistracted, and remember episodes of his life from long ago, but he was unable to form new long-term memories for facts and events. The pattern of H.M.'s impair ments also forced a re-examination of models of long-term memory storage. Specifically, although H.M. was able to retrieve long-term memories formed roughly a year or more before his surgery, he could not recall events that transpired within the year preceding his surgery. This strongly suggests that the transfer of information into long-term storage actually takes place over several years, with the hippocampus being necessary for its retrieval for the first year or so.
Subsequent research with other patients confirmed that the hippocampus, an irregularly shaped structure deep in the forebrain, is critically involved in the formation of memories for events (see figure 2 for a depiction of the brain, with the hippocampus and other rele vant structures highlighted). Patient R.B. lost a significant amount of blood as a result of heart surgery. He survived but showed memory impairments sim ilar to those exhibited by H.M. Upon his death, histology revealed that the loss of blood to R.B.'s brain damaged a small region of the hippocampus called hippocampal area CA1, which contains Alcohol and Memory neurons known as pyramidal cells because of the triangular shape of their cell bodies (Zola-Morgan et al. 1986). Hippocampal CA1 pyramidal cells assist the hippocampus in communi cating with other areas of the brain. The hippocampus receives information from a wide variety of brain regions, many of them located in the tissue, called the neocortex, that blankets the brain and surrounds other brain struc tures. (Neocortex literally means "new bark" or "new covering." When one looks at a picture of the human brain, most of what is visible is neocortex.) The hippocampus somehow ties infor mation from other brain regions together to form new autobiographical memories, and CA1 pyramidal cells send the results of this processing back out to the neocortex. As is clear from patient R.B., removing CA1 pyramidal cells from the circuitry prevents the hippocampal memory system from doing its job.
The second barrier to understanding the mechanisms underlying alcohol's effects on memory was an incomplete understanding of how alcohol affects brain function at a cellular level. Until recently, alcohol was assumed to affect the brain in a general way, simply shutting down the activity of all cells with which it came in contact. The Figure 2 The human brain, showing the location of the hippocampus, the frontal lobes, and the medial septum.

Medial septum
Hippocampus pervasiveness of this assumption is reflected in numerous writings during the early 20th century. For instance, Fleming (1935) wrote, "The prophetic generalization of Schmiedeberg in 1833 that the pharmacological action of alco hol on the cerebrum is purely depres sant has been found, most pharmacolo gists will agree, to characterize its action in general on all tissues" (p. 89). During the 1970s, researchers hypothesized that alcohol depressed neural activity by altering the movement of key mole cules (in particular, lipids) in nerve cell membranes. This change then led to alterations in the activity of proteins, including those that influence communication between neurons by controlling the passage of positively or negatively charged atoms (i.e., ions) through cell membranes (e.g., Chin and Goldstein 1977). This view per sisted into the late 1980s, at which time the consensus began to shift as evidence mounted that alcohol has selective effects on the brain's nerve-cell communication (i.e., neurotransmitter) systems, altering activity in some types of receptors but not others (e.g., Criswell et al. 1993). Substantial evi dence now indicates that alcohol selec tively alters the activity of specific com plexes of proteins embedded in the membranes of cells (i.e., receptors) that bind neurotransmitters such as gamma aminobutyric acid (GABA), glutamate, serotonin, acetylcholine, and glycine (for a review, see Little 1999). In some cases, only a few amino acids appear to distinguish receptors that are sensitive to alcohol from those that are not (Peoples and Stewart 2000). It remains unclear exactly how alcohol interacts with receptors to alter their activity.

Alcohol, Memory, and the Hippocampus
More than 30 years ago, both Ryback (1970) and Goodwin and colleagues (1969a) speculated that alcohol might impair memory formation by disrupt ing activity in the hippocampus. This speculation was based on the observation that acute alcohol exposure (in humans) produces a syndrome of memory impair ments similar in many ways to the impair ments produced by hippocampal damage. Specifically, both acute alcohol exposure and hippocampal damage impair the ability to form new long-term, explicit memories but do not affect short-term memory storage or, in general, the recall of information from long-term storage.
Research conducted in the past few decades using animal models supports the hypothesis that alcohol impairs memory formation, at least in part, by disrupting activity in the hippocampus (for a review, see White et al. 2000b). Such research has included behavioral observation; examination of slices of and brain tissue, neurons in cell culture, and brain activity in anesthetized or freely behaving animals; and a variety of pharmacological techniques.
As mentioned above, damage limited to the CA1 region of the hippocampus dramatically disrupts the ability to form new explicit memories (Zola-Morgan et al. 1986). In rodents, the actions of CA1 pyramidal cells have striking behavioral correlates. Some cells tend to discharge electrical signals that result in one cell communicating with other cells (i.e., action potentials) when the rodent is in a distinct location in its environment. The location differs for each cell. For instance, while a rat searches for food on a plus-shaped maze, one pyramidal cell might generate action potentials primarily when the rat is at the far end of the north arm, while Figure 3 Alcohol suppresses hippocampal pyramidal cell activity in an awake, freely behaving rat. Pyramidal cells often fire when the animal is in discrete regions of its environment, earning them the title "place-cells." The specific areas of the environ ment where these cells fire are referred to as place-fields. The figure shows the activity of an individual pyramidal cell before alcohol administration (baseline), 45 to 60 minutes after alcohol administration, and 7 hours after alcohol adminis tration (1.5 g/kg). Each frame in the figure shows the firing rate and firing location of the cell across a 15-minute block of time during which the rat was foraging for food on a symmetric, Y-shaped maze. White pixels are pixels in which the cell fired at very low rates, and darker colors represent higher firing rates (see key to the right of figure). As is clear from a comparison of activity during baseline and 45 to 60 minutes after alcohol administration, the activity of the cell was essen tially shut off by alcohol. Neural activity returned to near normal levels within roughly 7 hours after alcohol administration.

Alcohol and Memory
another might generate action potentials primarily when the rat is in the middle of the south arm, and so on. Collectively, the cells that are active in that particu lar environment create a spatial, or con textual map that serves as a framework for event memories created in that envi ronment. Because of the location-specific firing of these cells, they often are referred to as "place-cells," and the regions of the environment in which they fire are referred to as "place-fields" (for reviews, see Best and White 1998;Best et al. 2001). Given that CA1 pyramidal cells are critically important to the forma tion of memories for facts and events, and the clear behavioral correlates of their activity in rodents, it is possible to assess the impact of alcohol on hip pocampal output in an intact, fully functional brain by studying these cells.
In recent work with awake, freely behaving rats, White and Best (2000) showed that alcohol profoundly sup presses the activity of pyramidal cells in region CA1. The researchers allowed the rats to forage for food for 15 min utes in a symmetric, Y-shaped maze and measured the animals' hippocampal activ ity using tiny wires (i.e., microelectrodes) implanted in their brains. Figure 3 displays the activity of an individual CA1 pyrami dal cell. The activity-which corresponds to the middle portion of the lower left arm of the maze-is shown before alcohol administration (A), 45 to 60 minutes after alcohol administration (B), and 7 hours after alcohol administration (C). The dose of alcohol used in the testing session was 1.5 grams per kilogram of body weightenough to produce a peak BAC of about 0.16 percent. (A corresponding BAC in humans would be twice the legal driving limit in most States.) As the figure illus trates, the cell's activity was essentially shut off by alcohol. Neural activity returned to near-normal levels within about 7 hours of alcohol administration.
White and Best administered several doses of alcohol in this study, ranging from 0.5 g/kg to 1.5 g/kg. (Only one of the experiments is represented in figure 3.) They found that the dose affected the degree of pyramidal cell suppression. Although 0.5 g/kg did not produce a significant change in the firing of hip pocampal pyramidal cells, 1.0 and 1.5 g/kg produced significant suppression of firing during a 1-hour testing session following alcohol administration. The dose-dependent suppression of CA1 pyramidal cells is consistent with the dose-dependent effects of alcohol on episodic memory formation.

Alcohol and Hippocampal Long-Term Potentiation
In addition to suppressing the output from pyramidal cells, alcohol has several other effects on hippocampal function. For instance, alcohol severely disrupts the ability of neurons to establish longlasting, heightened responsiveness to signals from other cells (Bliss and Collinridge 1993). This heightened responsiveness is known as long-term potentiation (LTP). Because researchers have theorized that something like LTP occurs naturally in the brain during learning (for a review, see Martin and Morris 2002), many investigators have used LTP as a model for studying the neurobiology underlying the effects of drugs, including alcohol, on memory.
In a typical LTP experiment, two electrodes (A and B) are lowered into a slice of hippocampal tissue kept alive by bathing it in oxygenated artificial cere bral spinal fluid (ACSF). A small amount of current is passed through electrode A, causing the neurons in this area to send signals to cells located near electrode B. Electrode B then is used to record how the cells in the area respond to the incoming signals. This response is the baseline response. Next, a specific pat tern of stimulation intended to model the pattern of activity that might occur during an actual learning event is deliv ered through electrode A. When the original stimulus that elicited the base line response is delivered again through electrode A, the response recorded at electrode B is larger (i.e., potentiated). In other words, as a result of the pat terned input, cells at position B now are more responsive to signals sent from cells at position A. The potenti ated response often lasts for an extended period of time, hence the term long-term potentiation.
Alcohol interferes with the establish ment of LTP (Morrisett and Swartzwelder 1993;Givens and McMahon 1995;Pyapali et al. 1999;Schummers and Browning 2001), and this impairment begins at concentrations equivalent to those produced by consuming just one or two standard drinks (e.g., a 12-oz beer, 1.5-oz of liquor in a shot or mixed drink, or a 5-oz glass of wine) (Blitzer et al. 1990). If sufficient alcohol is pre sent in the ACSF bathing the slice of hippocampal tissue when the patterned stimulation is given, the response recorded later at position B will not be larger than it was at baseline (that is, it will not be potentiated). And, just as alcohol tends not to impair recall of memories established before alcohol exposure, alcohol does not disrupt the expression of LTP established before alcohol exposure.
One of the key requirements for the establishment of LTP in the hippocampus is that a type of signal receptor known as the NMDA 2 receptor becomes acti vated. Activation of the NMDA receptor allows calcium to enter the cell, which sets off a chain of events leading to long-lasting changes in the cell's structure or function, or both. Alcohol interferes with the activation of the NMDA receptor, thereby preventing the influx of calcium and the changes that follow (Swartzwelder et al. 1995). This is believed to be the primary mechanism underlying the effects of alcohol on LTP, though other transmitter systems probably are also involved (Schummers and Browning 2001).

Indirect Effects of Alcohol on Hippocampal Function
Like other brain regions, the hippocampus does not operate in isolation. Information processing in the hippocampus depends on coordinated input from a variety of other structures, which gives alcohol and other drugs additional opportuni ties to disrupt hippocampal function ing. One brain region that is central to hippocampal functioning is a small structure in the fore brain known as the medial septum (Givens et al. 2000). The medial septum sends rhythmic excitatory and inhibitory signals to the hippocampus, causing rhythmic changes in the activity of hippocampal pyramidal cells. In electroencephalograph record ings, this rhythmic activity, referred to as the theta rhythm, occurs within a frequency of roughly 6 to 9 cycles per second (hertz) in actively behaving rats. The theta rhythm is thought to act as a gatekeeper, increasing or decreasing the likelihood that information entering the hippocampus from cortical structures will be processed (Orr et al. 2001).
(For more information on the role of electrophysiology in diagnosing alcohol problems, see the article in this issue by Porjesz and Begleiter.) Information entering the hippocampus when pyrami dal cells are slightly excited (i.e., slightly depolarized) has a better chance of influ encing hippocampal circuitry than sig nals that arrive when the cells are slightly suppressed (i.e., slightly hyperpolarized).
Manipulations that disrupt the theta rhythm also disrupt the ability to perform tasks that depend on the hippocampus (Givens et al. 2000). Alcohol disrupts the theta rhythm in large part by sup pressing the output of signals from medial septal neurons to the hippocampus (Steffensen et al. 1993;Givens et al. 2000). Given the powerful influence that the medial septum has on infor mation processing in the hippocampus, the impact of alcohol on cellular activity in the medial septum is likely to play an important role in the effects of alcohol on memory. Indeed, in rats, putting alcohol directly into the medial septum alone produces memory impairments (Givens and McMahon 1997).

Other Brain Regions Involved in Alcohol-Induced Memory Impairments
The hippocampus is not the only struc ture involved in memory formation. A host of other brain structures also are involved in memory formation, storage, and retrieval (Eichenbaum 2002). Recent research with humans has yielded com pelling evidence that key areas of the frontal lobes play important roles in short-term memory and the formation and retrieval of long-term explicit memories (e.g., Shastri 2002;Curtis and D'Esposito 2003;Ranganath et al. 2003). Damage to the frontal lobes leads to profound cognitive impairments, one of which is a difficulty forming new memories. Recent evidence suggests that memory processes in the frontal lobes and the hippocampus are coordi nated via reciprocal connections (Wall and Messier 2001;Shastri 2002), rais ing the possibility that dysfunction in one structure could have deleterious effects on the functioning of the other.
Considerable evidence suggests that chronic alcohol use damages the frontal lobes and leads to impaired performance of tasks that rely on frontal lobe func tioning (Kril and Halliday 1999;Moselhy et al. 2001). "Shrinkage" in brain vol ume, changes in gene expression, and disruptions in how performing certain tasks affects blood flow in the brain all have been observed in the frontal lobes of alcohol-dependent subjects (Kril and Halliday 1999;Lewohl et al. 2000;Tapert et al. 2001;Kubota et al. 2001;Desmond et al. 2003).
Although much is known about the effects of chronic (i.e., repeated) use of alcohol on frontal lobe function, little is known about the effects of one-time (i.e., acute) use of alcohol on activity in the frontal lobes, or the relationship of such effects to alcohol-induced mem ory impairments. Compelling evidence indicates that acute alcohol use impairs the performance of a variety of frontal lobe-mediated tasks, like those that require planning, decisionmaking, and impulse control (Weissenborn and Duka 2003;Burian et al. 2003), but the underlying mechanisms are not known. Research also suggests that baseline blood flow to the frontal lobes increases during acute intoxication (Volkow et al. 1988;Tiihonen et al. 1994), that metabolism in the frontal lobes decreases , and that alcohol reduces the amount of activity that occurs in the frontal lobes when the frontal lobes are exposed to pulses from a strong mag netic field (Kahkonen et al. 2003). Although the exact meaning of these changes remains unclear, the evidence suggests that acute intoxication alters the normal functioning of the frontal lobes. Future research is needed to shed more light on this important question. In particular, research in animals will be an important supplement to studies in humans, affording a better understanding of the underlying prefrontal circuitry involved in alcohol-induced memory impairment.

Summary and Conclusions
As detailed in this brief review, alcohol can have a dramatic impact on mem ory. Alcohol primarily disrupts the abil ity to form new long-term memories; it causes less disruption of recall of previ ously established long-term memories or of the ability to keep new informa tion active in short-term memory for a few seconds or more. At low doses, the impairments produced by alcohol are often subtle, though they are detectable in controlled conditions. As the amount of alcohol consumed increases, so does the magnitude of the memory impair ments. Large quantities of alcohol, particularly if consumed rapidly, can produce a blackout, an interval of time for which the intoxicated person cannot recall key details of events, or even entire events. En bloc blackouts are stretches of time for which the person has no memory whatsoever. Fragmentary black outs are episodes for which the drinker's memory is spotty, with "islands" of mem ory providing some insight into what transpired, and for which more recall usually is possible if the drinker is cued by others. Blackouts are much more common among social drinkers than previously assumed and should be viewed as a potential consequence of acute intoxication regardless of age or whether one is clinically dependent upon alcohol.
Tremendous progress has been made toward an understanding of the mechanisms underlying alcohol-induced memory impairments. Alcohol disrupts activity in the hippocampus via several routes-directly, through effects on hippocampal circuitry, and indirectly, by interfering with interactions between the hippocampus and other brain regions. The impact of alcohol on the frontal lobes remains poorly understood, but probably plays an important role in alcohol-induced memory impairments.
Modern neuroimaging techniques, such as positron emission tomography (PET) and functional magnetic reso nance imaging (fMRI), provide incredi ble opportunities for investigating the impact of drugs like alcohol on brain function during the performance of cog nitive tasks. The use of these techniques will no doubt yield important informa tion regarding the mechanisms underly ing alcohol-induced memory impair ments in the coming years. Memory formation and retrieval are highly influ enced by factors such as attention and motivation (e.g., Kensinger et al. 2003). With the aid of neuroimaging tech niques, researchers may be able to exam ine the impact of alcohol on brain activ ity related to these factors, and then determine how alcohol contributes to memory impairments.
Despite advances in human neuro imaging techniques, animal models remain absolutely essential in the study of mechanisms underlying alcohol-induced memory impairments. Hopefully, future work will reveal more regarding the ways in which the effects of alcohol on multi ple transmitter systems interact to disrupt memory formation. Similarly, recent advances in electrophysiological recording techniques, which allow for recordings from hundreds of individual cells in sev eral brain regions simultaneously (Kralik et al. 2001), could provide much-needed information regarding the impact of alco hol on the interactions between disparate brain regions involved in the encoding, storage, and retrieval of information. ■